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anti tlr4 antibody  (Bioss)


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    Bioss anti tlr4 antibody
    AVA suppresses <t>TLR4</t> expression in rat colon. photomicrographs depicting immunohistochemical staining of TLR4 in rat colon. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4
    Anti Tlr4 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr4+polyclonal+antibody/TLR4+Polyclonal+Antibody/pmc13110223-93-6-10
    Average 93 stars, based on 50 article reviews
    anti tlr4 antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Therapeutic Repurposing of Avanafil Against Lipopolysaccharide-induced Depression and Autoimmune Hepatitis: Gut-brain-liver Axis Orchestration Via Regulation of TLR4/NF-κB/IDO and Nrf2/HO-1 Pathways"

    Article Title: Therapeutic Repurposing of Avanafil Against Lipopolysaccharide-induced Depression and Autoimmune Hepatitis: Gut-brain-liver Axis Orchestration Via Regulation of TLR4/NF-κB/IDO and Nrf2/HO-1 Pathways

    Journal: Molecular Neurobiology

    doi: 10.1007/s12035-026-05854-4

    AVA suppresses TLR4 expression in rat colon. photomicrographs depicting immunohistochemical staining of TLR4 in rat colon. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4
    Figure Legend Snippet: AVA suppresses TLR4 expression in rat colon. photomicrographs depicting immunohistochemical staining of TLR4 in rat colon. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Techniques Used: Expressing, Immunohistochemical staining, Staining, Control, Comparison

    AVA attenuates hepatic TLR4 expression. photomicrographs depicting immunohistochemical staining of TLR4 in rat liver. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4
    Figure Legend Snippet: AVA attenuates hepatic TLR4 expression. photomicrographs depicting immunohistochemical staining of TLR4 in rat liver. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Techniques Used: Expressing, Immunohistochemical staining, Staining, Control, Comparison

    Related Articles

    Western Blot:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino

    Immunofluorescence:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino

    Plasmid Preparation:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino

    Enzyme-linked Immunosorbent Assay:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino

    Blocking Assay:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino

    Incubation:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino

    Avidin-Biotin Assay:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino

    Activity Assay:

    Article Title: Indole-3-Acetic Acid: Promising Protective Agent Against Methotrexate-Induced Liver Injury via Modulation of TLR4/NF-κB/Caspase-3 Pathway
    Article Snippet: ELISA kits were purchased from Solarbio Life Sciences, Beijing, China. Primary polyclonal TLR4 rabbit (bs-20594R), NF-κB rabbit (ab16502), and caspase-3 rabbit (ab4051) antibodies were purchased from Bioss Antibodies Inc.® (Woburn, MA, USA). Biotinylated anti-rabbit IgG (H + L) (BA-1000) secondary antibody was purchased from Vector Laboratories, Inc. (Burlingame, CA, USA). Adult male Wistar albino



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    Image Search Results


    AVA suppresses TLR4 expression in rat colon. photomicrographs depicting immunohistochemical staining of TLR4 in rat colon. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Journal: Molecular Neurobiology

    Article Title: Therapeutic Repurposing of Avanafil Against Lipopolysaccharide-induced Depression and Autoimmune Hepatitis: Gut-brain-liver Axis Orchestration Via Regulation of TLR4/NF-κB/IDO and Nrf2/HO-1 Pathways

    doi: 10.1007/s12035-026-05854-4

    Figure Lengend Snippet: AVA suppresses TLR4 expression in rat colon. photomicrographs depicting immunohistochemical staining of TLR4 in rat colon. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Article Snippet: The cells were then treated with anti-TLR4 antibody (bs-1021R - Bioss USA - 1:200), anti-ZO-1(1:1000 - Abcam - EPR19945-224), and anti-MMP-9 (GTX100458 - GeneTex Inc. - 1:200) overnight at 4 °C.

    Techniques: Expressing, Immunohistochemical staining, Staining, Control, Comparison

    AVA attenuates hepatic TLR4 expression. photomicrographs depicting immunohistochemical staining of TLR4 in rat liver. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Journal: Molecular Neurobiology

    Article Title: Therapeutic Repurposing of Avanafil Against Lipopolysaccharide-induced Depression and Autoimmune Hepatitis: Gut-brain-liver Axis Orchestration Via Regulation of TLR4/NF-κB/IDO and Nrf2/HO-1 Pathways

    doi: 10.1007/s12035-026-05854-4

    Figure Lengend Snippet: AVA attenuates hepatic TLR4 expression. photomicrographs depicting immunohistochemical staining of TLR4 in rat liver. A Control group, B LPS group, C LPS+AVA-treated group, D AVA alone treatment, and E quantification of TLR4 area percentage. The data (mean ± SD) underwent statistical evaluation utilizing one-way ANOVA with Tukey’s post hoc comparison. Statistical significance was indicated by “a” and “b,” representing differences from the normal control and LPS groups, respectively, at P < 0.05. LPS, lipopolysaccharide; AVA, avanafil; TLR4, Toll-like receptor 4

    Article Snippet: The cells were then treated with anti-TLR4 antibody (bs-1021R - Bioss USA - 1:200), anti-ZO-1(1:1000 - Abcam - EPR19945-224), and anti-MMP-9 (GTX100458 - GeneTex Inc. - 1:200) overnight at 4 °C.

    Techniques: Expressing, Immunohistochemical staining, Staining, Control, Comparison

    Effect of FP and SFP on the TLR4/MyD88 signaling in blood vessels. (A) Representative images from immunohistochemical staining of TLR4 in blood vessels (×200). (B, C, E) Western blotting to determine the levels of TLR4, MyD88, NFκB p65 and its phosphorylation, IκBα and its phosphorylation, and IKKβ and its phosphorylation in blood vessels. (D) Changes in serum TNF-α and IL-6 levels. (F) Relative expression of TLR4, MyD88, NFκB p65, IκBα, and IKKβ mRNA. Compared with the WKY group, # p < 0.05, ## p < 0.01; compared with the SHR group, * p < 0.05, ** p < 0.01; compared with the FP group, ▲ p < 0.05, ▲▲ p < 0.01, n = 3.

    Journal: Ultrasonics Sonochemistry

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy

    doi: 10.1016/j.ultsonch.2026.107829

    Figure Lengend Snippet: Effect of FP and SFP on the TLR4/MyD88 signaling in blood vessels. (A) Representative images from immunohistochemical staining of TLR4 in blood vessels (×200). (B, C, E) Western blotting to determine the levels of TLR4, MyD88, NFκB p65 and its phosphorylation, IκBα and its phosphorylation, and IKKβ and its phosphorylation in blood vessels. (D) Changes in serum TNF-α and IL-6 levels. (F) Relative expression of TLR4, MyD88, NFκB p65, IκBα, and IKKβ mRNA. Compared with the WKY group, # p < 0.05, ## p < 0.01; compared with the SHR group, * p < 0.05, ** p < 0.01; compared with the FP group, ▲ p < 0.05, ▲▲ p < 0.01, n = 3.

    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).

    Techniques: Immunohistochemical staining, Staining, Western Blot, Phospho-proteomics, Expressing